Nature is a recurring motif and material in Giuseppe Penone’s oeuvre. This article discusses how Penone’s way of experimenting with the relationship between artistic intentionality and the processuality of natural materials reflects the desire in contemporary posthuman theory to renounce the modern hierarchy between human and non-human entities. Reading Penone’s practice in light of posthuman theory demonstrates how his approach to nature challenges the established comprehension of ‘art as representation’ and the connotations imbedded in the concept of an ‘artwork’.